Sperm Motility

Sperm Motility

Sperm concentration assessment (M/ml) and motility analysis are performed on native samples by recording AVI clips (acquired live from your imaging device to PC memory or prerecorded to disk). The analysis strictly follows the requirements of the “WHO laboratory manual for the examination of human semen and sperm-cervical mucus interaction”. The analysis is based on frame by frame detection of sperm heads on video clips and building precise tracks which reveal the nature of sperm movement and provide sperm concentration value. The total number of sperms is also calculated automatically. One frame with tracks is automatically saved to database record to serve as visual support in the report or you can save a suitable frame yourself.

The following parameters are calculated:

Pointing with your mouse cursor to a track, you can see the exact values of the parameters for the selected spermatozoon and classification result in a pop-up window. The clip can be played again, rewound, played frame by frame for detailed analysis. You can save clips to your hard disc uncompressed without loss of quality or by using virtually any codec installed on your PC (xVid codec is recommended). You can adjust detection threshold and also change the classification limits if required.

Based on the parameters mentioned above, the motility of each spermatozoon is graded A, B, C or D (WHO), according to whether it shows:

Additionally, the software enables the user to assess the concentration of:

Flexible tool for adjustment of sperm head detection will let you work with both bright field and phase contrast (negative phase contrast yields the best results) and not only human sperms but also many animal species.